Hotspot

psbM-trnD-GUC

LSC · 32616-33395 · 780 bp

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Pi 0.00278
MarkerSeek score 60.10
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
780
variable_sites
4
pi
0.00278
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 ATGAACAGCGCAGTAGCAAT TTCAATTGGTCAGAGCACCG 45.0 50.0 58.3 58.5 886-888 bp 1.0 74.7
psbM-trnD-GUC_p2 TGAACAGCGCAGTAGCAATA TTCAATTGGTCAGAGCACCG 45.0 50.0 57.6 58.5 885-887 bp 1.0 73.7
psbM-trnD-GUC_p3 AATGAACAGCGCAGTAGCAA TTCAATTGGTCAGAGCACCG 45.0 50.0 58.5 58.5 887-889 bp 1.0 72.9
psbM-trnD-GUC_p4 AGAATGAACAGCGCAGTAGC TTCAATTGGTCAGAGCACCG 50.0 50.0 58.6 58.5 889-891 bp 1.0 71.6
psbM-trnD-GUC_p5 ACTAGAATGAACAGCGCAGT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.2 58.5 892-894 bp 1.0 70.7