Hotspot

rps16-trnQ-UUG

LSC · 6040-8216 · 2177 bp

Back to Nuphar

Pi 0.00541
MarkerSeek score 62.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
2177
variable_sites
21
pi
0.00541
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 2251-2276 bp 1.0 80.8
rps16-trnQ-UUG_p2 GTCATTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 2234-2259 bp 1.0 80.6
rps16-trnQ-UUG_p3 ACGTTGCCTTCTACCACATC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 2220-2245 bp 1.0 80.4
rps16-trnQ-UUG_p4 GTTGCCTTCTACCACATCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 2218-2243 bp 1.0 80.4
rps16-trnQ-UUG_p5 CATGTCATTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 2237-2262 bp 1.0 79.9