Hotspot

trnK-UUU-rps16

LSC · 4147-4947 · 801 bp

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Pi 0.0125
MarkerSeek score 52.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
801
variable_sites
19
pi
0.0125
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG CAAAAGGTATGTTGCTGCCG 55.0 50.0 58.0 58.0 1614-1647 bp 1.0 81.3
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG CAAAAGGTATGTTGCTGCCG 55.0 50.0 58.0 58.0 1613-1646 bp 1.0 81.3
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG GGATCCGTTTGCTATCGGTT 55.0 50.0 58.0 58.1 1274-1307 bp 1.0 81.1
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG GGATCCGTTTGCTATCGGTT 55.0 50.0 58.0 58.1 1273-1306 bp 1.0 81.1
trnK-UUU-rps16_p5 TCCCTTTCAGGATCAGTCGT CAAAAGGTATGTTGCTGCCG 50.0 50.0 58.1 58.0 1615-1648 bp 1.0 81.0