Hotspot

ycf1

SSC · 122613-127619 · 5007 bp

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Pi 0.000999

MarkerSeek score 53.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5007
variable_sites: 5
pi: 0.000999
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCGGCTCCCATTATTGGTAT	GTCTAGACGCCGTAGAGAAT	50.0	50.0	57.8	56.3	259 bp	1.0	65.0
ycf1_p2	AACATACTAAAGCGGCTCCC	GTCTAGACGCCGTAGAGAAT	50.0	50.0	57.7	56.3	270 bp	1.0	64.0
ycf1_p3	GCGGCTCCCATTATTGGTAT	GTCTAGACGCCGTAGAGAATT	50.0	47.6	57.8	57.0	259 bp	1.0	63.0
ycf1_p4	CATACTAAAGCGGCTCCCAT	GTCTAGACGCCGTAGAGAAT	50.0	50.0	57.4	56.3	268 bp	1.0	62.2
ycf1_p5	AACATACTAAAGCGGCTCCC	GTCTAGACGCCGTAGAGAATT	50.0	47.6	57.7	57.0	270 bp	1.0	61.9