Hotspot

rps16

LSC · 5167-6292 · 1126 bp

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Pi 0.000888

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1126
variable_sites: 1
pi: 0.000888
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAGCAAGAAAAGAAGGAAA	TCTCTGTACCGACTGAACCA	35.0	50.0	52.6	57.7	1321-1322 bp	1.0	40.1
rps16_p2	AGAGCAAGAAAAGAAGGAAAT	TCTCTGTACCGACTGAACCA	33.3	50.0	53.3	57.7	1321-1322 bp	1.0	40.1
rps16_p3	AGAGCAAGAAAAGAAGGAAA	GTCTCTGTACCGACTGAACC	35.0	55.0	52.6	57.4	1322-1323 bp	1.0	40.1
rps16_p4	TCGAAATCACTATCCTATCCT	TCTCTGTACCGACTGAACCA	38.1	50.0	53.0	57.7	1261-1262 bp	1.0	40.1
rps16_p5	AGAGCAAGAAAAGAAGGAAAT	GTCTCTGTACCGACTGAACC	33.3	55.0	53.3	57.4	1322-1323 bp	1.0	40.1