Hotspot

petA

LSC · 61557-62519 · 963 bp

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Pi 0.00104

MarkerSeek score 59.30

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	TTCGGGATCAATGGTTGGAC	TTGAGGTCGATAAATCCGCG	50.0	50.0	57.9	58.2	1007 bp	1.0	77.9
petA_p2	TTCGGGATCAATGGTTGGAC	GAGGTCGATAAATCCGCGAG	50.0	55.0	57.9	58.3	1005 bp	1.0	76.6
petA_p3	TTTCGGGATCAATGGTTGGA	TTGAGGTCGATAAATCCGCG	45.0	50.0	57.1	58.2	1008 bp	1.0	71.7
petA_p4	TTCGGGATCAATGGTTGGAC	AGGTCGATAAATCCGCGAGT	50.0	50.0	57.9	59.0	1004 bp	1.0	71.3
petA_p5	TTCGGGATCAATGGTTGGAC	TGAGGTCGATAAATCCGCGA	50.0	50.0	57.9	59.0	1006 bp	1.0	71.3