Hotspot

ndhG

SSC · 117080-117610 · 531 bp

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Pi 0.00188

MarkerSeek score 58.40

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 1
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AGGAAATGCATGTGATAGCTCA	TTTTTCCTGACCGGGTCAAT	40.9	45.0	57.8	57.3	650 bp	1.0	57.5
ndhG_p2	AGGAAATGCATGTGATAGCTCA	CCTGACCGGGTCAATAAAGT	40.9	50.0	57.8	57.2	645 bp	1.0	56.6
ndhG_p3	AGGAAATGCATGTGATAGCTCA	CCTGACCGGGTCAATAAAGTT	40.9	47.6	57.8	57.9	645 bp	1.0	54.3
ndhG_p4	AGGAAATGCATGTGATAGCTCA	TCCTGACCGGGTCAATAAAG	40.9	50.0	57.8	56.9	646 bp	1.0	54.3
ndhG_p5	GGAAATGCATGTGATAGCTCA	TTTTTCCTGACCGGGTCAAT	42.9	45.0	56.4	57.3	649 bp	1.0	53.8