Hotspot

clpP

LSC · 69422-71456 · 2035 bp

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Pi 0

MarkerSeek score 43.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2035
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	2953 bp	1.0	79.4
clpP_p2	TGACCCTGTTAGTCCGTTCT	GGACCCCCTCTTTAGTGGAT	50.0	55.0	58.0	58.1	2968 bp	1.0	79.2
clpP_p3	CTGAACGAGTCGCACATACA	GGACCCCCTCTTTAGTGGAT	50.0	55.0	58.0	58.1	2693 bp	1.0	79.1
clpP_p4	ATGATCTGAACGAGTCGCAC	ACGAACATACCCTGTCTCCA	50.0	50.0	58.1	58.1	2958 bp	1.0	78.9
clpP_p5	GATCTGAACGAGTCGCACAT	ACGAACATACCCTGTCTCCA	50.0	50.0	58.1	58.1	2956 bp	1.0	78.9