Hotspot

atpF

LSC · 26781-28052 · 1272 bp

Back to Notoseris

Pi 0.000786

MarkerSeek score 59.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1272
variable_sites: 1
pi: 0.000786
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACAAAAAGAACTCTGTTCGA	ATTTCGTCGGCTTGAATGGT	35.0	45.0	53.0	58.2	1412 bp	1.0	40.1
atpF_p2	ACAAAAAGAACTCTGTTCGA	GTCGGCTTGAATGGTTACCA	35.0	50.0	53.0	58.2	1407 bp	1.0	40.1
atpF_p3	ACAAAAAGAACTCTGTTCGA	TCGTCGGCTTGAATGGTTAC	35.0	50.0	53.0	58.3	1409 bp	1.0	40.1
atpF_p4	ACAAAAAGAACTCTGTTCGA	TTTCGTCGGCTTGAATGGTT	35.0	45.0	53.0	58.4	1411 bp	1.0	40.1
atpF_p5	ACAAAAAGAACTCTGTTCGA	TTCGTCGGCTTGAATGGTTA	35.0	45.0	53.0	57.5	1410 bp	1.0	40.1