Hotspot

atpB-rbcL

LSC · 54118-54885 · 768 bp

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Pi 0.0013

MarkerSeek score 59.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 768
variable_sites: 1
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	928-929 bp	1.0	71.8
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	927-928 bp	1.0	71.5
atpB-rbcL_p3	CGCCCCAGTTTCTTTTTGTC	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	881-882 bp	1.0	71.0
atpB-rbcL_p4	CGCCCCAGTTTCTTTTTGTC	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	880-881 bp	1.0	70.7
atpB-rbcL_p5	TGGTAACCCCAGAACCAGAA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.2	58.0	856-857 bp	1.0	70.6