Hotspot

petB

LSC · 75361-76753 · 1393 bp

Back to Nothosmyrnium

Pi 0.00574

MarkerSeek score 48.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1393
variable_sites: 8
pi: 0.00574
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AACAGCTCCTTTGATGGGTG	ATGCGGCAGATGGATAAAGG	50.0	50.0	58.1	58.1	1876 bp	1.0	79.0
petB_p2	AACAGCTCCTTTGATGGGTG	CGGCAGATGGATAAAGGCAT	50.0	50.0	58.1	58.1	1873 bp	1.0	79.0
petB_p3	AACAGCTCCTTTGATGGGTG	TATGCGATGGTTGGAGCAAA	50.0	45.0	58.1	57.9	1924 bp	1.0	78.8
petB_p4	AACAGCTCCTTTGATGGGTG	TTATGCGATGGTTGGAGCAA	50.0	45.0	58.1	57.9	1925 bp	1.0	78.8
petB_p5	AACAGCTCCTTTGATGGGTG	TTCCAATGTAATGCGGCAGA	50.0	45.0	58.1	57.9	1886 bp	1.0	78.8