Hotspot

ndhH-rps15

SSC · 125040-125130 · 91 bp

Back to Nothosmyrnium

Pi 0.022

MarkerSeek score 65.10

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 91
variable_sites: 2
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	GGTCATTTCGTGTAGCTGGT	CGGACAGTTGGAGATTCGAG	50.0	55.0	57.9	58.1	156 bp	1.0	79.6
ndhH-rps15_p2	ATGAGTCGAAGAACACCGTG	CGGACAGTTGGAGATTCGAG	50.0	55.0	57.9	58.1	214 bp	1.0	79.5
ndhH-rps15_p3	ATGAGTCGAAGAACACCGTG	CTAGGAAAACGTCAACGGCT	50.0	50.0	57.9	58.2	282 bp	1.0	78.4
ndhH-rps15_p4	ATGAGTCGAAGAACACCGTG	TCTAGGAAAACGTCAACGGC	50.0	50.0	57.9	58.2	283 bp	1.0	78.3
ndhH-rps15_p5	GGTCATTTCGTGTAGCTGGT	CTAGGAAAACGTCAACGGCT	50.0	50.0	57.9	58.2	224 bp	1.0	78.3