Hotspot

rpl32-trnL-UAG

SSC · 115240-116178 · 939 bp

Back to Nothosmyrnium

Pi 0.0129
MarkerSeek score 69.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
939
variable_sites
11
pi
0.0129
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGATATTGGGCAGCCTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 985-1063 bp 1.0 83.5
rpl32-trnL-UAG_p2 AGGGATATTGGGCAGCCTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 986-1064 bp 1.0 83.5
rpl32-trnL-UAG_p3 AGGGATATTGGGCAGCCTTA CTCGCACTGCTTCCTAAGAG 50.0 55.0 58.2 58.1 1006-1084 bp 1.0 82.9
rpl32-trnL-UAG_p4 AGGGATATTGGGCAGCCTTA CTCTCGCACTGCTTCCTAAG 50.0 55.0 58.2 58.1 1008-1086 bp 1.0 82.9
rpl32-trnL-UAG_p5 AGGGATATTGGGCAGCCTTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 995-1073 bp 1.0 82.5