Hotspot

trnL-CAA-ndhB

LSC · 93860-94430 · 571 bp

Back to Nothosmyrnium

Pi 0.016

MarkerSeek score 64.10

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: LSC
length_bp: 571
variable_sites: 9
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	624-631 bp	1.0	81.5
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	622-629 bp	1.0	81.5
trnL-CAA-ndhB_p3	TACCATTTCACCACCAAGGC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.1	57.9	612-619 bp	1.0	81.0
trnL-CAA-ndhB_p4	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	619-626 bp	1.0	80.0
trnL-CAA-ndhB_p5	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	623-630 bp	1.0	79.4