Hotspot

rps11-rpl36

LSC · 79845-79957 · 113 bp

Back to Nothosmyrnium

Pi 0.027

MarkerSeek score 66.90

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 3
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CCTTTGGTATTCGCCGTGTA	ATTTGTTCCAACCCAAGACA	50.0	40.0	58.0	55.3	232-234 bp	1.0	60.4
rps11-rpl36_p2	CCTTTGGTATTCGCCGTGTA	TTTGTTCCAACCCAAGACAT	50.0	40.0	58.0	55.3	231-233 bp	1.0	60.4
rps11-rpl36_p3	CGCCGTGTATTCTTACGTGA	ATTTGTTCCAACCCAAGACA	50.0	40.0	58.1	55.3	221-223 bp	1.0	59.8
rps11-rpl36_p4	CGCCGTGTATTCTTACGTGA	TTTGTTCCAACCCAAGACAT	50.0	40.0	58.1	55.3	220-222 bp	1.0	59.8
rps11-rpl36_p5	TCGCCGTGTATTCTTACGTG	TTTGTTCCAACCCAAGACAT	50.0	40.0	58.1	55.3	221-223 bp	1.0	59.8