Hotspot

petA-psbJ

LSC · 63855-64496 · 642 bp

Back to Nothosmyrnium

Pi 0.0358

MarkerSeek score 71.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 642
variable_sites: 23
pi: 0.0358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTTATTTTGGCACA	GCTGGTATTCTTGTGATCGGT	42.9	47.6	57.4	58.1	802 bp	1.0	60.8
petA-psbJ_p2	TGGCATCTGTTATTTTGGCAC	GCTGGTATTCTTGTGATCGGT	42.9	47.6	57.4	58.1	803 bp	1.0	60.8
petA-psbJ_p3	GGCATCTGTTATTTTGGCACA	AGCTGGTATTCTTGTGATCGG	42.9	47.6	57.4	57.8	803 bp	1.0	60.1
petA-psbJ_p4	TGGCATCTGTTATTTTGGCAC	AGCTGGTATTCTTGTGATCGG	42.9	47.6	57.4	57.8	804 bp	1.0	60.1
petA-psbJ_p5	TTGGCATCTGTTATTTTGGCAC	GCTGGTATTCTTGTGATCGGT	40.9	47.6	58.1	58.1	804 bp	1.0	56.9