Hotspot

petN-psbM

LSC · 29110-30001 · 892 bp

Back to Nothosmyrnium

Pi 0.0135

MarkerSeek score 66.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 12
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATCC	50.0	50.0	58.2	57.9	1066-1325 bp	1.0	78.8
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATCC	50.0	50.0	57.6	57.9	1005-1264 bp	1.0	77.2
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	GCTACTGCACTGTTCATCCT	50.0	50.0	58.2	57.6	1065-1324 bp	1.0	76.4
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	GCTACTGCACTGTTCATCCT	50.0	50.0	57.6	57.6	1004-1263 bp	1.0	74.9
petN-psbM_p5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATCC	50.0	50.0	57.1	57.9	1067-1326 bp	1.0	72.9