Hotspot

trnG-GCC-trnR-UCU

LSC · 9797-9935 · 139 bp

Back to Nothosmyrnium

Pi 0.0153
MarkerSeek score 65.80
Primer available yes
Label trnG-GCC-trnR-UCU

Feature Metadata

label_name
trnG-GCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
139
variable_sites
2
pi
0.0153
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 212-220 bp 1.0 67.1
trnG-GCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 215-223 bp 1.0 66.8
trnG-GCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 218-226 bp 1.0 66.3
trnG-GCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 221-229 bp 1.0 66.0
trnG-GCC-trnR-UCU_p5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.7 56.9 216-224 bp 1.0 64.6