Hotspot

rpl33

LSC · 69153-69353 · 201 bp

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Pi 0

MarkerSeek score 22.10

Primer available yes

Label rpl33

Feature Metadata

label_name: rpl33
feature_type: gene
region: LSC
length_bp: 201
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33_p1	CGTCTTCTTAGGTTAGGGTCG	AGATCATACACACGTTCCATTCA	52.4	39.1	57.6	57.9	289 bp	1.0	44.0
rpl33_p2	CGTCTTCTTAGGTTAGGGTCG	TGAAAGATCATACACACGTTCCA	52.4	39.1	57.6	58.1	293 bp	1.0	43.8
rpl33_p3	CGTCTTCTTAGGTTAGGGTCG	TCATACACACGTTCCATTCA	52.4	40.0	57.6	54.7	286 bp	1.0	42.4
rpl33_p4	CGTCTTCTTAGGTTAGGGTCG	TCATACACACGTTCCATTCAA	52.4	38.1	57.6	55.5	286 bp	1.0	41.0
rpl33_p5	CGTCTTCTTAGGTTAGGGTCG	GATCATACACACGTTCCATTCA	52.4	40.9	57.6	56.5	288 bp	1.0	40.9