Hotspot

ndhD

SSC · 117561-119063 · 1503 bp

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Pi 0.00266

MarkerSeek score 60.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 4
pi: 0.00266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTTTTGCGTTGTACAGCGAA	CAAAAGCCCGTACTCGAGAA	40.0	50.0	57.2	57.9	1658 bp	1.0	73.0
ndhD_p2	TTTTTGCGTTGTACAGCGAA	TTACCGACAAAAGCCCGTAC	40.0	50.0	57.2	58.2	1665 bp	1.0	71.9
ndhD_p3	TTTGCGTTGTACAGCGAATT	CAAAAGCCCGTACTCGAGAA	40.0	50.0	57.0	57.9	1656 bp	1.0	71.2
ndhD_p4	TTTTGCGTTGTACAGCGAAT	CAAAAGCCCGTACTCGAGAA	40.0	50.0	57.0	57.9	1657 bp	1.0	71.2
ndhD_p5	TTTTTGCGTTGTACAGCGAA	TTTTACCGACAAAAGCCCGT	40.0	45.0	57.2	58.3	1667 bp	1.0	71.0