Hotspot

rps15-ycf1

SSC · 125624-126133 · 510 bp

Back to Nothoscordum

Pi 0.0118

MarkerSeek score 52.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 510
variable_sites: 6
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TTTTCTTGTCGAATCGCGGA	AGGAAAAGTATCTTAATACAGATGCA	45.0	30.8	58.2	56.5	598-599 bp	1.0	40.9
rps15-ycf1_p2	GTCGAATCGCGGAGATGAAT	AGGAAAAGTATCTTAATACAGATGCA	50.0	30.8	58.2	56.5	591-592 bp	1.0	40.9
rps15-ycf1_p3	CGAATCGCGGAGATGAATGA	AGGAAAAGTATCTTAATACAGATGCA	50.0	30.8	58.2	56.5	589-590 bp	1.0	40.9
rps15-ycf1_p4	TCGAATCGCGGAGATGAATG	AGGAAAAGTATCTTAATACAGATGCA	50.0	30.8	58.2	56.5	590-591 bp	1.0	40.9
rps15-ycf1_p5	TTTTCTTGTCGAATCGCGGA	GGAAAAGTATCTTAATACAGATGCA	45.0	32.0	58.2	55.2	597-598 bp	1.0	40.9