Hotspot

ndhF-rpl32

SSC · 114179-115167 · 989 bp

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Pi 0.00616
MarkerSeek score 57.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
989
variable_sites
6
pi
0.00616
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTGGGAATAAGCAGAAGTCCA TGACTTTGCTAAAGAAAAAGCT 47.6 31.8 57.6 54.8 1191-1192 bp 1.0 41.4
ndhF-rpl32_p2 TGTGGGAATAAGCAGAAGTCC TGACTTTGCTAAAGAAAAAGCT 47.6 31.8 57.6 54.8 1192-1193 bp 1.0 41.4
ndhF-rpl32_p3 GTGGGAATAAGCAGAAGTCCA TGACTTTGCTAAAGAAAAAGCTT 47.6 30.4 57.6 55.5 1191-1192 bp 1.0 41.4
ndhF-rpl32_p4 GTGGGAATAAGCAGAAGTCCA TTGACTTTGCTAAAGAAAAAGCT 47.6 30.4 57.6 55.5 1192-1193 bp 1.0 41.4
ndhF-rpl32_p5 TGTGGGAATAAGCAGAAGTCC TGACTTTGCTAAAGAAAAAGCTT 47.6 30.4 57.6 55.5 1192-1193 bp 1.0 41.4