Hotspot

petA-psbJ

LSC · 64501-65622 · 1122 bp

Back to Nothoscordum

Pi 0.0098

MarkerSeek score 64.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1122
variable_sites: 11
pi: 0.0098
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.7	58.1	1306 bp	1.0	74.2
petA-psbJ_p2	CGTCCAAGGCCTTTTGTTCT	TTCCTATTCCGGGTTGGGTT	50.0	50.0	58.7	58.6	1262 bp	1.0	70.2
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.7	57.3	1258 bp	1.0	69.5
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	CTATTCCGGGTTGGGTTCAT	50.0	50.0	58.7	57.3	1259 bp	1.0	69.4
petA-psbJ_p5	TCCAAGGCCTTTTGTTCTTCT	GCTGGTATTCCTGTGATCGG	42.9	55.0	57.7	58.1	1304 bp	1.0	69.1