Hotspot

rbcL-accD

LSC · 58339-59144 · 806 bp

Back to Nothoscordum

Pi 0.00372

MarkerSeek score 60.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 3
pi: 0.00372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGCTGCTTGTGAAGTATGGA	AATGGGCTCTTCACTTCCAC	50.0	50.0	58.3	57.8	968 bp	1.0	76.4
rbcL-accD_p2	CGCTGCTTGTGAAGTATGGA	GAATGGGCTCTTCACTTCCA	50.0	50.0	58.3	57.5	969 bp	1.0	74.1
rbcL-accD_p3	CGCTGCTTGTGAAGTATGGA	TGCCCAACAGCATTAAGACT	50.0	45.0	58.3	57.4	943 bp	1.0	73.4
rbcL-accD_p4	CGCTGCTTGTGAAGTATGGA	CTTCACTTCCACCGGTATGC	50.0	55.0	58.3	58.6	960 bp	1.0	73.0
rbcL-accD_p5	CGCTGCTTGTGAAGTATGGA	AGAATGGGCTCTTCACTTCC	50.0	50.0	58.3	57.2	970 bp	1.0	71.7