Hotspot

psaA-ycf3

LSC · 42585-43204 · 620 bp

Back to Nothoscordum

Pi 0.00162

MarkerSeek score 60.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 1
pi: 0.00162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTTCTTGAAAAATGGCCGGG	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.9	57.9	833-837 bp	1.0	79.1
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.9	57.9	808-812 bp	1.0	78.9
psaA-ycf3_p3	GTTCTTGAAAAATGGCCGGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	747-751 bp	1.0	78.6
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	722-726 bp	1.0	78.5
psaA-ycf3_p5	GTTCTTGAAAAATGGCCGGG	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	751-755 bp	1.0	78.4