Hotspot

ndhD

SSC · 113762-115267 · 1506 bp

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Pi 0.00299

MarkerSeek score 35.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 9
pi: 0.00299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGTTCGCAAAAACTCGAGA	ACGGGCTTTTCTGGTCAAAG	45.0	50.0	58.1	58.7	1630 bp	1.0	74.5
ndhD_p2	TGGTTCGCAAAAACTCGAGA	CACGGGCTTTTCTGGTCAAA	45.0	50.0	58.1	59.0	1631 bp	1.0	72.2
ndhD_p3	TGGTTCGCAAAAACTCGAGA	CACGGGCTTTTCTGGTCAA	45.0	52.6	58.1	58.3	1631 bp	1.0	69.7
ndhD_p4	TGGTTCGCAAAAACTCGAGA	GCTTTTCTGGTCAAAGCGTA	45.0	45.0	58.1	56.7	1626 bp	1.0	69.2
ndhD_p5	TGGTTCGCAAAAACTCGAGA	ACGGGCTTTTCTGGTCAAA	45.0	47.4	58.1	57.5	1630 bp	1.0	67.9