Hotspot

matK

LSC · 1696-3237 · 1542 bp

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Pi 0.00389

MarkerSeek score 55.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1542
variable_sites: 12
pi: 0.00389
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCAATCATTTGGAAATTGGATCA	CCTGTTCGGACCATATTGCA	30.4	50.0	54.8	58.0	1738-1743 bp	1.0	40.6
matK_p2	TCAATCATTTGGAAATTGGATCA	AAGAAATGCCCCCTGTCTTG	30.4	50.0	54.8	58.1	1683-1688 bp	1.0	40.6
matK_p3	TCAATCATTTGGAAATTGGATCA	CAAGAAATGCCCCCTGTCTT	30.4	50.0	54.8	58.1	1684-1689 bp	1.0	40.6
matK_p4	TCAATCATTTGGAAATTGGATCA	ATAACCCAAGAAATGCCCCC	30.4	50.0	54.8	57.8	1690-1695 bp	1.0	40.6
matK_p5	TGTCTATCAATCATTTGGAAATTGGA	CCTGTTCGGACCATATTGCA	30.8	50.0	57.4	58.0	1744-1749 bp	1.0	40.5