Hotspot

psaC-ndhE

SSC · 115640-116034 · 395 bp

Back to Notholirion

Pi 0.019

MarkerSeek score 45.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 395
variable_sites: 15
pi: 0.019
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CATAAATTTTTACTGAATGTGACA	ACTCGTATCAATCAATCAAATTTGT	25.0	28.0	52.3	55.9	455-456 bp	1.0	42.8
psaC-ndhE_p2	CATAAATTTTTACTGAATGTGACA	ACTCGTATCAATCAATCAAATTTGTTG	25.0	29.6	52.3	57.7	455-456 bp	1.0	42.8
psaC-ndhE_p3	TCATAAATTTTTACTGAATGTGAC	ACTCGTATCAATCAATCAAATTTGT	25.0	28.0	52.1	55.9	456-457 bp	1.0	42.8
psaC-ndhE_p4	CATAAATTTTTACTGAATGTGACA	TCGTATCAATCAATCAAATTTGTTGA	25.0	26.9	52.3	56.5	453-454 bp	1.0	42.8
psaC-ndhE_p5	CATAAATTTTTACTGAATGTGACA	ACTCGTATCAATCAATCAAATTTGTT	25.0	26.9	52.3	56.5	455-456 bp	1.0	42.8