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rps12-clpP

LSC · 67966-68118 · 153 bp

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Pi 0.0142

MarkerSeek score 58.10

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 4
pi: 0.0142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CGGGGGATTTCGTGACATTT	GCATTGTTGATCTTGTAGCGG	50.0	47.6	58.5	57.9	266-273 bp	1.0	70.5
rps12-clpP_p2	CGGGGGATTTCGTGACATTT	GGCATTGTTGATCTTGTAGCG	50.0	47.6	58.5	57.9	267-274 bp	1.0	70.5
rps12-clpP_p3	CGGGGGATTTCGTGACATTT	CCCATGGCATTGTTGATCTTG	50.0	47.6	58.5	57.8	272-279 bp	1.0	69.5
rps12-clpP_p4	GTACATGTTCCTCGACGCTG	GCATTGTTGATCTTGTAGCGG	55.0	47.6	58.7	57.9	303-310 bp	1.0	68.6
rps12-clpP_p5	GTACATGTTCCTCGACGCTG	GGCATTGTTGATCTTGTAGCG	55.0	47.6	58.7	57.9	304-311 bp	1.0	68.5