Hotspot

trnC-GCA-petN

LSC · 26982-27529 · 548 bp

Back to Notholirion

Pi 0.00395

MarkerSeek score 42.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 548
variable_sites: 4
pi: 0.00395
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CAAATCTGGGTGTCGCCTAA	CTCCTCCCCCATACTACGAG	50.0	60.0	57.9	58.1	656-657 bp	1.0	79.0
trnC-GCA-petN_p2	CAAATCTGGGTGTCGCCTAA	ACGAGCGAAAGGGAAAATGT	50.0	45.0	57.9	58.1	641-642 bp	1.0	78.9
trnC-GCA-petN_p3	CAAATCTGGGTGTCGCCTAA	CCATACTACGAGCGAAAGGG	50.0	55.0	57.9	57.9	648-649 bp	1.0	78.7
trnC-GCA-petN_p4	CAAATCTGGGTGTCGCCTAA	CCCATACTACGAGCGAAAGG	50.0	55.0	57.9	57.9	649-650 bp	1.0	78.7
trnC-GCA-petN_p5	CAAATCTGGGTGTCGCCTAA	CCCCATACTACGAGCGAAAG	50.0	55.0	57.9	57.9	650-651 bp	1.0	78.7