Hotspot

rpoB-trnC-GCA

LSC · 26240-26900 · 661 bp

Back to Notholirion

Pi 0.00857

MarkerSeek score 58.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 661
variable_sites: 11
pi: 0.00857
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GCCAAGCCCCGATTAATGAA	TTAGGCGACACCCAGATTTG	50.0	50.0	58.6	57.9	829-843 bp	1.0	75.5
rpoB-trnC-GCA_p2	GCCAAGCCCCGATTAATGAA	TTGTCAATTAGGCGACACCC	50.0	50.0	58.6	58.2	836-850 bp	1.0	74.9
rpoB-trnC-GCA_p3	GCCAAGCCCCGATTAATGAA	CAATTAGGCGACACCCAGAT	50.0	50.0	58.6	57.7	832-846 bp	1.0	73.7
rpoB-trnC-GCA_p4	ATGAAATTCTTCCGCCAAGC	TTAGGCGACACCCAGATTTG	45.0	50.0	57.1	57.9	842-856 bp	1.0	72.7
rpoB-trnC-GCA_p5	GCCAAGCCCCGATTAATGAA	GCGACACCCAGATTTGAACT	50.0	50.0	58.6	58.5	825-839 bp	1.0	72.5