Hotspot

psbK-psbI

LSC · 7125-7260 · 136 bp

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Pi 0.00746

MarkerSeek score 57.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 136
variable_sites: 2
pi: 0.00746
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	GGATTGCGTCCTGGATCATT	50.0	50.0	57.9	58.0	253-261 bp	1.0	82.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGTCCAGGATTGC	50.0	50.0	57.9	58.2	266-274 bp	1.0	81.0
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	GGATTGCGTCCTGGATCATT	45.0	50.0	58.3	58.0	250-258 bp	1.0	80.5
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	GGATTGCGTCCTGGATCATT	45.0	50.0	58.3	58.0	251-259 bp	1.0	80.4
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	GGATTGCGTCCTGGATCATT	45.0	50.0	58.3	58.0	252-260 bp	1.0	80.4