Hotspot

ycf1

SSC · 124785-130415 · 5631 bp

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Pi 0.00237

MarkerSeek score 54.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5631
variable_sites: 20
pi: 0.00237
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCATACCATTCAGGAAGTTGCA	GAGGGGGTCAGCATTCAAAA	40.9	50.0	57.7	58.1	255-279 bp	1.0	66.5
ycf1_p2	ACCATTCAGGAAGTTGCAAT	GAGGGGGTCAGCATTCAAAA	40.0	50.0	55.5	58.1	251-275 bp	1.0	64.9
ycf1_p3	TCAGGAAGTTGCAATAGTAGCA	GAGGGGGTCAGCATTCAAAA	40.9	50.0	57.4	58.1	246-270 bp	1.0	64.5
ycf1_p4	TCATACCATTCAGGAAGTTGCA	AGAAATTGGGTAGGGAGGGG	40.9	55.0	57.7	58.4	269-293 bp	1.0	63.9
ycf1_p5	CATACCATTCAGGAAGTTGCA	GAGGGGGTCAGCATTCAAAA	42.9	50.0	56.2	58.1	254-278 bp	1.0	62.7