Hotspot

ndhF

SSC · 111806-114022 · 2217 bp

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Pi 0.000902

MarkerSeek score 44.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 3
pi: 0.000902
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.0	58.0	2628 bp	1.0	71.7
ndhF_p2	GAGATCCGCCGAAGAAGATC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	57.9	58.0	2492 bp	1.0	71.5
ndhF_p3	CAGGAACAAGAGAGATCCGC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	58.1	58.0	2503 bp	1.0	71.5
ndhF_p4	CGCCGAAGAAGATCCTTCTC	ATGCTGTCCCATACCCTTTC	55.0	50.0	58.2	57.3	2996 bp	0.333333	46.2
ndhF_p5	GCCGAAGAAGATCCTTCTCC	ATGCTGTCCCATACCCTTTC	55.0	50.0	57.8	57.3	2995 bp	0.333333	45.6