Hotspot

ccsA

SSC · 115369-116340 · 972 bp

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Pi 0.00206

MarkerSeek score 45.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 3
pi: 0.00206
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTTCAAGACAAGCCGCTA	TCAGTAGCTTTTCGATGGCC	50.0	50.0	57.9	58.0	1778-1779 bp	1.0	79.4
ccsA_p2	GAGTTCAAGACAAGCCGCTA	CAGTAGCTTTTCGATGGCCT	50.0	50.0	57.9	58.0	1777-1778 bp	1.0	79.3
ccsA_p3	TAGACACGCTGCTCTTAGGA	TCAGTAGCTTTTCGATGGCC	50.0	50.0	57.9	58.0	1746-1747 bp	1.0	79.0
ccsA_p4	ACAAGCCGCTATGGTGAAAT	TCAGTAGCTTTTCGATGGCC	45.0	50.0	57.9	58.0	1769-1770 bp	1.0	79.0
ccsA_p5	TAGACACGCTGCTCTTAGGA	CAGTAGCTTTTCGATGGCCT	50.0	50.0	57.9	58.0	1745-1746 bp	1.0	79.0