Hotspot

atpF

LSC · 12425-13687 · 1263 bp

Back to Notelaea

Pi 0.000528

MarkerSeek score 60.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1263
variable_sites: 1
pi: 0.000528
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGCTCGAATGGTTACCATGA	AGGCAAAGTGATACAAAAAGAACT	50.0	33.3	57.7	57.3	1392-1405 bp	1.0	40.7
atpF_p2	GGCTCGAATGGTTACCATGA	GGCAAAGTGATACAAAAAGAACTC	50.0	37.5	57.7	57.0	1391-1404 bp	1.0	40.7
atpF_p3	ACTAATTTCGTCGGCTCGAA	AGGCAAAGTGATACAAAAAGAACT	45.0	33.3	57.4	57.3	1404-1417 bp	1.0	40.7
atpF_p4	GGCTCGAATGGTTACCATGA	AGGCAAAGTGATACAAAAAGAAC	50.0	34.8	57.7	55.9	1392-1405 bp	1.0	40.7
atpF_p5	GGCTCGAATGGTTACCATGA	GGCAAAGTGATACAAAAAGAACT	50.0	34.8	57.7	55.9	1391-1404 bp	1.0	40.7