Hotspot

ndhE-ndhG

SSC · 119015-119215 · 201 bp

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Pi 0.00663

MarkerSeek score 56.70

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 201
variable_sites: 2
pi: 0.00663
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCGACTTGTGATCAATCCGT	GCAATTGCAATAGCTCGTCA	45.0	45.0	57.0	57.2	300 bp	1.0	65.6
ndhE-ndhG_p2	TTCGACTTGTGATCAATCCGT	GCAATTGCAATAGCTCGTCA	42.9	45.0	57.7	57.2	301 bp	1.0	63.4
ndhE-ndhG_p3	TCGACTTGTGATCAATCCGT	GCAATTGCAATAGCTCGTCAA	45.0	42.9	57.0	57.9	300 bp	1.0	63.2
ndhE-ndhG_p4	TTCGACTTGTGATCAATCCGT	GCAATTGCAATAGCTCGTCAA	42.9	42.9	57.7	57.9	301 bp	1.0	60.9
ndhE-ndhG_p5	GACACATAAGGGCTCTAACCA	GCAATTGCAATAGCTCGTCA	47.6	45.0	57.1	57.2	325 bp	1.0	58.6