Hotspot

ndhF-rpl32

SSC · 114023-114495 · 473 bp

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Pi 0.00282

MarkerSeek score 35.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 473
variable_sites: 2
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TGATGCAGAAGTACGTTTTT	50.0	35.0	58.0	53.5	604-607 bp	1.0	43.7
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	TTGATGCAGAAGTACGTTTT	50.0	35.0	58.0	53.5	605-608 bp	1.0	43.7
ndhF-rpl32_p3	GTTGCCGTCGGAAAAAGAAG	TTTGATGCAGAAGTACGTTT	50.0	35.0	58.0	53.5	606-609 bp	1.0	43.7
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	TTTTGATGCAGAAGTACGTT	50.0	35.0	58.0	53.5	607-610 bp	1.0	43.7
ndhF-rpl32_p5	GTTGCCGTCGGAAAAAGAAG	TTTTTGATGCAGAAGTACGT	50.0	35.0	58.0	53.5	608-611 bp	1.0	43.7