Hotspot

atpB-rbcL

LSC · 56081-56871 · 791 bp

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Pi 0.0118

MarkerSeek score 67.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 14
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGGCCCAGGTTTTGTTT	AACACCCGCTTTGAATCCAA	45.0	45.0	58.0	58.3	904-910 bp	1.0	79.4
atpB-rbcL_p2	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	875-881 bp	1.0	78.8
atpB-rbcL_p3	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	946-952 bp	1.0	78.1
atpB-rbcL_p4	TACGGCCCAGGTTTTGTTTT	AACACCCGCTTTGAATCCAA	45.0	45.0	58.2	58.3	903-909 bp	1.0	77.7
atpB-rbcL_p5	GGACCGATGATTTGGACGAT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.8	58.3	922-928 bp	1.0	77.5