Hotspot

ndhC-trnM(CAU)

LSC · 52116-53907 · 1792 bp

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Pi 0.00112
MarkerSeek score 58.90
Primer available yes
Label ndhC-trnM(CAU)

Feature Metadata

label_name
ndhC-trnM(CAU)
feature_type
igs
region
LSC
length_bp
1792
variable_sites
3
pi
0.00112
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM(CAU)_p1 GGGGCTAAAATCCCGGAAAT CCCGCCGTATGAAAGCAATA 50.0 50.0 57.9 58.4 1934 bp 1.0 76.2
ndhC-trnM(CAU)_p2 GGGGCTAAAATCCCGGAAAT AGGTACCATTGACTCCGGTA 50.0 50.0 57.9 57.5 1995 bp 1.0 75.2
ndhC-trnM(CAU)_p3 GGGGCTAAAATCCCGGAAAT TAGGTACCATTGACTCCGGT 50.0 50.0 57.9 57.5 1996 bp 1.0 75.2
ndhC-trnM(CAU)_p4 GGGGCTAAAATCCCGGAAAT CCGCCGTATGAAAGCAATAC 50.0 50.0 57.9 57.3 1933 bp 1.0 74.2
ndhC-trnM(CAU)_p5 TTCTCCGGCCCTTTCCTAAT CCCGCCGTATGAAAGCAATA 50.0 50.0 58.4 58.4 1955 bp 1.0 73.6