Hotspot

petN-psbM

LSC · 29783-30948 · 1166 bp

Back to Notelaea

Pi 0.00457

MarkerSeek score 61.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 8
pi: 0.00457
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	GCGACTGCACTATTCATTCT	50.0	45.0	58.0	55.9	1275 bp	1.0	64.0
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	GCGACTGCACTATTCATTCT	50.0	45.0	58.0	55.9	1273 bp	1.0	64.0
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	GCGACTGCACTATTCATTCT	55.0	45.0	57.9	55.9	1272 bp	1.0	63.4
petN-psbM_p4	TAGTGTGGGGAAGAAGTGGA	GCGACTGCACTATTCATTCT	50.0	45.0	57.6	55.9	1270 bp	1.0	60.8
petN-psbM_p5	CCTGGGCTGCTTTAATGGTA	GCGACTGCACTATTCATTCT	50.0	45.0	57.6	55.9	1315 bp	1.0	60.6