Hotspot

trnH(GUG)-psbA

LSC · 94-538 · 445 bp

Back to Notelaea

Pi 0.00451
MarkerSeek score 59.10
Primer available yes
Label trnH(GUG)-psbA

Feature Metadata

label_name
trnH(GUG)-psbA
feature_type
igs
region
LSC
length_bp
445
variable_sites
3
pi
0.00451
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-psbA_p1 TTAGTTATGGGCGAACGACG CCGTGCTAACCTTGGTATGG 50.0 55.0 58.1 58.3 622-625 bp 1.0 77.4
trnH(GUG)-psbA_p2 CTGCCTTAATCCACTTGGCT CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 567-570 bp 1.0 77.1
trnH(GUG)-psbA_p3 TTAGTTATGGGCGAACGACG ACCGTGCTAACCTTGGTATG 50.0 50.0 58.1 57.6 623-626 bp 1.0 76.9
trnH(GUG)-psbA_p4 ACTGCCTTAATCCACTTGGC CCGTGCTAACCTTGGTATGG 50.0 55.0 58.2 58.3 568-571 bp 1.0 76.9
trnH(GUG)-psbA_p5 CTGCCTTAATCCACTTGGCT ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 568-571 bp 1.0 76.6