Hotspot

rps2

LSC · 16698-17408 · 711 bp

Back to Noronhia

Pi 0.000563

MarkerSeek score 29.30

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.000563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ACGTGAGTAAAGCCAAAGCA	GGTCGGATCGATTGGTTGAA	45.0	50.0	58.0	58.0	1828-1834 bp	1.0	80.5
rps2_p2	GGAAACCCCCTATTTGCCAA	AGTGCCCTCCTCAAAACAAG	50.0	50.0	58.1	58.0	1412-1417 bp	0.8	72.5
rps2_p3	TTGGAAACCCCCTATTTGCC	AGTGCCCTCCTCAAAACAAG	50.0	50.0	58.1	58.0	1414-1419 bp	0.8	72.5
rps2_p4	ACGTGAGTAAAGCCAAAGCA	AGTGCCCTCCTCAAAACAAG	45.0	50.0	58.0	58.0	1745-1750 bp	0.8	72.4
rps2_p5	ACGTGAGTAAAGCCAAAGCA	GTGCCCTCCTCAAAACAAGA	45.0	50.0	58.0	58.0	1744-1749 bp	0.8	72.4