Hotspot

rpl32-trnL(UAG)

SSC · 114923-115780 · 858 bp

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Pi 0.00583
MarkerSeek score 59.50
Primer available yes
Label rpl32-trnL(UAG)

Feature Metadata

label_name
rpl32-trnL(UAG)
feature_type
igs
region
SSC
length_bp
858
variable_sites
11
pi
0.00583
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL(UAG)_p1 TGGGACAGCGTTAAAAGCAT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 978-991 bp 1.0 79.6
rpl32-trnL(UAG)_p2 TGGGACAGCGTTAAAAGCAT AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 971-984 bp 1.0 79.1
rpl32-trnL(UAG)_p3 TGGGACAGCGTTAAAAGCAT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.1 57.8 976-989 bp 1.0 79.1
rpl32-trnL(UAG)_p4 TGGGACAGCGTTAAAAGCAT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.1 57.8 977-990 bp 1.0 79.0
rpl32-trnL(UAG)_p5 TGGGACAGCGTTAAAAGCAT GAGCAGCGTGTCTACCAATT 45.0 50.0 58.1 58.3 972-985 bp 1.0 78.4