Hotspot

rps19-rpl2

LSC · 86391-86455 · 65 bp

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Pi 0.0231

MarkerSeek score 66.90

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 3
pi: 0.0231
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TTTTTAGTGAACGTGCCAC	TTGATTCTTCGTCGCCGTAG	42.1	50.0	54.2	58.1	105-112 bp	1.0	46.8
rps19-rpl2_p2	TTTTTAGTGAACGTGCCAC	ATTTGATTCTTCGTCGCCGT	42.1	45.0	54.2	58.3	107-114 bp	1.0	46.2
rps19-rpl2_p3	TTTTTAGTGAACGTGCCAC	TTTGATTCTTCGTCGCCGTA	42.1	45.0	54.2	57.6	106-113 bp	1.0	46.2
rps19-rpl2_p4	TTTTTAGTGAACGTGCCAC	TTGGGGTTATCCTGCACTTG	42.1	50.0	54.2	57.8	163-170 bp	1.0	44.8
rps19-rpl2_p5	TTTTTAGTGAACGTGCCAC	CACAACCCCTTGGGGTTATC	42.1	55.0	54.2	58.2	172-179 bp	1.0	44.7