Hotspot

psbE-petL

LSC · 67161-68262 · 1102 bp

Back to Noronhia

Pi 0.00644

MarkerSeek score 56.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 16
pi: 0.00644
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CGTATCTTGCTCAGACCAAT	50.0	45.0	57.9	55.3	1278-1292 bp	1.0	59.9
psbE-petL_p2	ACTGACGAATAACCAACCCG	CGTATCTTGCTCAGACCAAT	50.0	45.0	57.9	55.3	1291-1305 bp	1.0	59.6
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	CGTATCTTGCTCAGACCAAT	50.0	45.0	57.8	55.3	1201-1215 bp	1.0	59.2
psbE-petL_p4	ACGAATAACCAACCCGCAAT	CGTATCTTGCTCAGACCAAT	45.0	45.0	58.2	55.3	1287-1301 bp	1.0	58.9
psbE-petL_p5	CGAATAACCAACCCGCAATG	CGTATCTTGCTCAGACCAAT	50.0	45.0	57.8	55.3	1286-1300 bp	1.0	58.8