Hotspot

psaA-ycf3

LSC · 43495-44228 · 734 bp

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Pi 0.00409

MarkerSeek score 62.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 7
pi: 0.00409
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	889-895 bp	1.0	80.0
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	841-847 bp	1.0	79.4
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCAGCTGA	50.0	45.0	57.9	57.7	914-920 bp	1.0	78.1
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGGCTATAGCGCTTACTCCT	50.0	50.0	57.9	57.7	883-889 bp	1.0	78.0
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TTGGAAACAGGCTATAGCGC	50.0	50.0	57.9	58.3	891-897 bp	1.0	77.6