Hotspot

rps2-rpoC2

LSC · 17409-17611 · 203 bp

Back to Noronhia

Pi 0.0286

MarkerSeek score 73.20

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 203
variable_sites: 13
pi: 0.0286
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AACTCCTGCCTCCATCATCT	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.1	57.8	374-380 bp	1.0	80.7
rps2-rpoC2_p2	TGCCTCCATCATCTCTTCCA	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.1	57.8	368-374 bp	1.0	80.7
rps2-rpoC2_p3	TCCTGCCTCCATCATCTCTT	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.8	57.8	371-377 bp	1.0	80.1
rps2-rpoC2_p4	GAACTCCTGCCTCCATCATC	TTCGAGGGGGAAATGAGAGA	55.0	50.0	57.7	57.8	375-381 bp	1.0	79.6
rps2-rpoC2_p5	CTCCTGCCTCCATCATCTCT	TTCGAGGGGGAAATGAGAGA	55.0	50.0	58.3	57.8	372-378 bp	1.0	79.4