Hotspot

atpA-atpF

LSC · 12738-12801 · 64 bp

Back to Noronhia

Pi 0.0297

MarkerSeek score 68.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 4
pi: 0.0297
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ACTAATTTCGTCGGCTCGAA	TTGGGGGCGATGAAAGAAAT	45.0	45.0	57.4	57.8	123-134 bp	1.0	76.7
atpA-atpF_p2	AATTTCGTCGGCTCGAATGG	TTGGGGGCGATGAAAGAAAT	50.0	45.0	59.0	57.8	120-131 bp	1.0	74.0
atpA-atpF_p3	ACTAATTTCGTCGGCTCGAA	GTTGGGGGCGATGAAAGAAA	45.0	50.0	57.4	58.7	124-135 bp	1.0	72.6
atpA-atpF_p4	ACTAATTTCGTCGGCTCGAAT	TTGGGGGCGATGAAAGAAAT	42.9	45.0	57.8	57.8	123-134 bp	1.0	72.6
atpA-atpF_p5	ACTAATTTCGTCGGCTCGAA	AATATTGGCATGTTGGGGGC	45.0	50.0	57.4	58.9	135-146 bp	1.0	71.3