Hotspot

psaB

LSC · 36666-38870 · 2205 bp

Back to Nordenstamia

Pi 0.00242

MarkerSeek score 44.60

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 8
pi: 0.00242
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	GCTACCAAACTGCTCTACCC	GTACAAGGACGTGCTGTAGG	55.0	55.0	58.0	58.0	2936 bp	1.0	80.1
psaB_p2	AAGGTTATGAGCCTTGCGAG	GTACAAGGACGTGCTGTAGG	50.0	55.0	58.0	58.0	2955 bp	1.0	80.0
psaB_p3	GCTACCAAACTGCTCTACCC	ACAACATGGGCGTTCTTCTT	55.0	45.0	58.0	58.0	2885 bp	1.0	80.0
psaB_p4	TTTGAACCCGTGACCTCAAG	GTACAAGGACGTGCTGTAGG	50.0	55.0	58.0	58.0	2972 bp	1.0	80.0
psaB_p5	AAGGTTATGAGCCTTGCGAG	ACAACATGGGCGTTCTTCTT	50.0	45.0	58.0	58.0	2904 bp	1.0	79.9